DNA POLYMERASES HAVING IMPROVED LABELED NUCLEOTIDE INCORPORATION PROPERTIES
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United States of America Patent
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Issued Date -
Aug 20, 2015
app pub date -
Jan 16, 2015
filing date -
Mar 12, 1997
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Abandoned
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Abstract
The present invention relates to mutant DNA polymerases that exhibit reduced discrimination against labeled nucleotides into polynucleotides. The DNA polymerases of the invention have at least one mutation in the nucleotide label interaction region of the enzyme such the mutation results in reduced discrimination against labeled nucleotides. The nucleotide label interaction regions is located at portions of the O-helix, (ii) the K helix, and (iii) the inter O-P helical loop of Taq DNA polymerase or analogous positions in other DNA polymerases.
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