Detection and Quantification of Hydroxymethylated Nucleotides in a Polynucleotide Preparation
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United States of America Patent
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Issued Date -
Aug 27, 2015
app pub date -
Apr 14, 2015
filing date -
Aug 25, 2009
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Abandoned
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Abstract
Methods and compositions are described for detecting hydroxymethylated nucleotides (hmNs) in a polynucleotide preparation with a view to mapping the location of hmNs in a genome, quantifying the occurrence of hmNs at selected loci and correlating the occurrence of hmNs with gene expression and phenotypic traits. Embodiments describe the use of modifying enzymes together with site-specific endonucleases to detect the hmNs.
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