Methods Of Producing A Secreted Protein
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United States of America Patent
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Issued Date -
Sep 3, 2015
app pub date -
Jan 9, 2015
filing date -
May 11, 2007
priority date (Note) -
Abandoned
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Abstract
The invention is directed to methods of producing a polypeptide or a variant thereof, wherein the polypeptide or variant thereof is dependent on LIMP-2 for trafficking, localization, stabilization and/or sorting of the polypeptide in the cell. In general, the methods comprise culturing a lysosomal integral membrane protein II (LIMP-2) deficient cell which expresses the polypeptide or the variant thereof under conditions in which the polypeptide or the variant thereof is produced.
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