HIGH RESOLUTION MICROSCOPY BY MEANS OF STRUCTURED ILLUMINATION AT LARGE WORKING DISTANCES

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United States of America Patent

APP PUB NO 20150260978A1
SERIAL NO

14431956

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Abstract

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A method for obtaining a sub-resolution image of a specimen using a microscope is provided. The method includes projecting an illumination pattern of illumination light onto the specimen, thereby illuminating the specimen, at least one of detecting at least a portion of fluorescent light emitted from the specimen and detecting at least a portion of illumination light reflected from the specimen, thereby capturing a series of images of the specimen at a plurality of different relative positions of the specimen with respect to the illumination pattern projected onto the specimen, wherein between the capturing of at least two images of the series the relative position of the specimen with respect to the illumination pattern projected onto the specimen is shifted in a non-controlled manner, and processing the captured images to extract a sub-resolution image of the specimen.

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Patent OwnerAddress
UNIVERSITAT HEIDELBERG69117 HEIDELBERG

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Amberger, Roman Bad Reichenhall, DE 3 28
Best, Gerrit Heidelberg, DE 3 34
Cremer, Christoph Heidelberg, DE 17 398
Heintzmann, Rainer Jena, DE 6 207

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