THERMO-RESPONSIVE LECTIN-ELP FUSION BINDING LIGANDS FOR GLYCOPROTEIN PURIFICATION BY AFFINITY PRECIPITATION

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United States of America Patent

APP PUB NO 20150307546A1
SERIAL NO

14699203

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Abstract

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The invention provides novel affinity fusion ligands, and method of use thereof, for glycoprotein purification by affinity precipitation. The affinity fusion ligand of the invention comprises a bacterial lectin, e.g., from Ralstonia solanacearum (RSL) that binds to fucose, and/or from the Vibrio cholera neuraminidase (VCNA) that binds to saialic acid, fused with a thermo-responsive polypeptide, e.g., an elastin-like polypeptides (ELP) repeats. Methods of using the lectin-ELP fusion ligand for purifying a glycoprotein via affinity precipitation are also provided herewith.

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Patent Owner(s)

  • GEORGIA TECH RESEARCH CORPORATION

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Arnold, Lindsay Grace Atlanta, US 1 0
Chen, Rachel Ruizhen Atlanta, US 4 8

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