TWO-PRIMER PCR FOR MICRORNA MULTIPLEX ASSAY
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United States of America Patent
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Nov 12, 2015
app pub date -
Sep 25, 2013
filing date -
Sep 28, 2012
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Abstract
The present invention provides method for amplifying a specific RNA molecule in a sample, the method comprising: (a) adding a poly(ribonucleotide) sequence to RNA molecules in the sample; (b) reverse transcribing the poly-adenylated RNA molecules using a reverse primer comprising a sequence that anneals to said poly(ribonucleotide) sequence; and (c) amplifying and detecting the cDNA molecule(s) using the same reverse primer and using a forward primer specific for the RNA molecule to be detected; wherein at least one of the forward and reverse primers comprises a hairpin primer. The invention also provides kits useful for practicing this method.
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