Rationally-Designed Single-Chain Meganucleases With Non-Palindromic Recognition Sequences
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United States of America Patent
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Nov 26, 2015
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May 28, 2015
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Oct 31, 2007
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Abstract
Disclosed are rationally-designed, non-naturally-occurring meganucleases in which a pair of enzyme subunits having specificity for different recognition sequence half-sites are joined into a single polypeptide to form a functional heterodimer with a non-palindromic recognition sequence. The invention also relates to methods of producing such meganucleases, and methods of producing recombinant nucleic acids and organisms using such meganucleases.
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