Gene-matched enrichment and polymerase chain reaction for rapid detection of microorganisms

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United States of America Patent

PATENT NO 10100369
APP PUB NO 20150368696A1
SERIAL NO

14742367

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Abstract

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A method for amplifying and detecting microorganisms, such as species of Listeria, is described. The method utilizes gene-matched enrichment media and PCR-based detection. The enrichment media is spent media produced using a modified microorganism containing a plurality of mutations in a selected gene such that the modified microorganism does not contain the PCR signature. Thus, PCR detects only the amplified microorganism of interest, not the modified microorganism. Exemplary methods and kits for amplification and detection of Listeria species are described.

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Patent Owner(s)

  • BATTELLE MEMORIAL INSTITUTE

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Hess, Becky M Richland, US 3 0
Hutchison, Janine R Richland, US 3 6
Ozanich, Richard M Richland, US 7 191
Victry, Kristin D Richland, US 2 1

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