Importance

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Abstract

The invention relates to a method for operating a light microscope, which comprises at least the following components: a modulator diaphragm for restricting a light cross-section, a sample plane, which is located in a beam path behind the modulator diaphragm and in which a sample can be positioned, a phase ring, which is arranged in the beam path behind the sample plane and along the cross-section of which incident light can be differently influenced, and a pupil camera for capturing a pupil image. The method involves the following steps: with the aid of a beam splitter, which is located in the beam path in front of the phase ring, light that emanates from the sample is guided in part to the pupil camera and in part to the phase ring; a pupil image, which is not influenced by the phase ring, is recorded and a sample image is simultaneously emitted; with the aid of electronic image processing means in the recorded pupil image, the position and size of an image of the modulator diaphragm, which is influenced by a sample but not influenced by the phase ring, is measured; a relative displacement between the phase ring and the modulator diaphragm is performed on the basis of the measured position and of the measured size of the image of the modulator diaphragm with the aid of electronic control means. The invention further relates to an corresponding optical arrangement.

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First Claim