METHOD FOR INTRODUCING GENE TO EUGLENA, AND TRANSFORMANT THEREFROM
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United States of America Patent
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Issued Date -
Jan 14, 2016
app pub date -
Feb 28, 2014
filing date -
Feb 28, 2013
priority date (Note) -
Abandoned
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Abstract
The present invention provides a method for introducing a gene into Euglena, which can stably maintain a foreign gene, and a transformant therefrom. In this method of introducing a gene into Euglena, a DNA fragment containing an amino acid sequence for encoding a protein is introduced into a Euglena cell. The method includes a step of producing a binary vector containing a DNA fragment, a step of obtaining a linear gene fragment that includes a T-DNA region including the DNA fragment in the binary vector, and a direct gene introduction step of directly introducing the linear gene fragment into the Euglena cell.
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