QUANTIFICATION OF A FUNCTIONALIZED SURFACE
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United States of America Patent
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Jan 14, 2016
app pub date -
Apr 25, 2014
filing date -
Apr 25, 2013
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Abstract
A method for quantifying at a functional group functionalizing a surface. First, the method comprising the step of providing at a surface comprised on a microparticle having micrometric dimensions functionalized with at a functional group. The method includes a step of contacting the surface with a solution: Said solution comprises at a first reagent at a first concentration and a second reagent at a second concentration, the ratio between the first concentration and the second concentration being predetermined. Said first reagent and second reagent are designed for selectively coupling to a functional group with respective affinities, the ratio of said affinities being predetermined. The first reagent is labelled for emitting fluorescence and the second reagent is un-labelled. Then, the fluorescence of said first reagent on the surface to quantify said functional group therefrom by using the measured fluorescence of said first reagent, and the predetermined ratios.
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