MICROFLUIDIC DEVICE FOR DETECTING NUCLEIC ACIDS AND ASSOCIATED METHODS
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United States of America Patent
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Jan 21, 2016
app pub date -
Jul 15, 2015
filing date -
Jul 15, 2014
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Abstract
Described is a device that is able to distinguish different nucleic acid concentrations in whole blood or plasma sample directly in under 10 min. By using this device, cell-free DNA in whole blood or plasma can be quantified without extensive sample preparation. The device contains a sample channel, an accumulation channel and a pair of electrodes. Optionally, the device includes power supply connected to the electrodes for generating an electric field to move the DNA from the sample channel to the collection channel, and a sensor to quantify the amount of cell free DNA in the collection channel. Also provided are methods for providing a prognosis for a subject with sepsis or suspected of having sepsis comprising quantifying nucleic acid concentrations in a sample from a subject using a device as described herein.
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