RAPID ISOLATION OF MONOCLONAL ANTIBODIES FROM ANIMALS
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United States of America Patent
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Feb 4, 2016
app pub date -
Jul 27, 2015
filing date -
May 17, 2010
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Abstract
Methods and compositions for identification of candicate antigen-specific variable regions as well as generation of antibodies or antigen-binding fragments that could have desired antigen specificity are provided. For example, in certain aspects methods for determining amino acid sequences of serum antibody CDR and abundancy level are described. In some aspects, methods for determining nucleic acid sequences of antibody variable region sequences and frequency are provided. Furthermore, the invention provides methods for identification and generation of antibody or antigen-binding fragments that comprise highly-represented CDR.
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