COMPOSITIONS AND METHODS FOR DETECTION OF A TARGET IN A MOLECULAR ASSAY USING PH CHANGES
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United States of America Patent
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Issued Date -
Feb 18, 2016
app pub date -
Aug 18, 2015
filing date -
Aug 18, 2014
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Abstract
The disclosure provides a sensor for detecting a target comprising a probe that is able to recognize the presence of the target; a pH-changing enzyme conjugated to an oligonucleotide that senses the recognition of the presence of the target by the probe; and a solid support linked or linkable to the probe; wherein the presence of the target causes the sensor to be either captured to the solid support or released into solution for detection of pH changes. Also provided are methods for using the sensor and kits.
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