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Abstract

The present invention provides a more productive method for culturing and a more productive method for harvesting culture product in cell culture wherein the cell produces the culture product. The present invention relates to a method for harvesting a culture product contained in a culture solution in the cell culture wherein the cell produces the culture product, comprising the following steps: B sending the culture solution to a filtration membrane; C: filtering the culture solution by alternating tangential flow filtration while changing the flow of the culture solution so as to cause a reciprocating motion thereof in a direction parallel with the surface of the filtration membrane to obtain a filtrate; D: sending back a culture solution residue that has remained without permeating the filtration membrane; and G: harvesting the culture product from the filtrate, wherein

the filtration membrane used in the step B is a porous membrane having an average pore size of 20 μm or larger and 100 μm or smaller for the pores in the surface of the culture solution side or a porous membrane in which the ratio of pores having a diameter smaller than 20 μm for the pores in the surface of the culture solution side is 50% or less of all pores in the surface of the culture solution side.

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First Claim