METHODS AND APPARATUS FOR HIGH-THROUGHPUT LABEL-FREE CELL ASSAY
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United States of America Patent
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Issued Date -
Apr 21, 2016
app pub date -
Apr 7, 2015
filing date -
Apr 17, 2014
priority date (Note) -
Abandoned
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Abstract
The present invention relates to a high-throughput label-free cell assay system which scans a lower part of medium made of glass using an object lens and a Galvano mirror and using a broadband laser as a light source, analyzes and visualizes light reflected from the lower part of the medium using a spectrometer, measures interference spectra formed by the light reflected from each interface of the medium using the spectrometer, and measures phase from data that the measured interference spectra were converted by Fourier transform to observe structural change of cells. The system includes a transparent medium on which a sample is put, an object lens located beneath the transparent medium; a Galvano mirror located beneath the object lens; an optical fiber coupler transferring light from the super-continuum light source to the Galvano mirror; and the spectrometer detecting spectrum of the light from the optical fiber coupler.
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