IN VITRO DETECTION OF PRIONS IN BLOOD
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United States of America Patent
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Issued Date -
Apr 28, 2016
app pub date -
Oct 22, 2015
filing date -
Oct 22, 2014
priority date (Note) -
Abandoned
status (Latency Note)
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Abstract
A method of screening a blood sample for the presence of prions. The method includes the steps of collecting the blood sample in heparin, contacting the sample with a solution comprising recombinant prion protein (rPrP) and Thioflavin T (ThT), and measuring the resulting ThT fluorescence in the sample. The method can further include the step of freezing and thawing the sample prior to contacting the sample with a solution comprising recombinant prion protein (rPrP) and Thioflavin T (ThT). The method can also include the step of precipitating the prions in sodium phosphotungstic acid (NaPTA) prior to contacting the sample with a solution comprising recombinant prion protein (rPrP) and Thioflavin T (ThT).
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