Proteomic identification of antibodies
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United States of America Patent
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Jan 8, 2019
Grant Date -
May 26, 2016
app pub date -
Sep 25, 2015
filing date -
May 17, 2010
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Abstract
Methods and compositions for identification of candidate antigen-specific variable regions as well as generation of antibodies or antigen-binding fragments that could have desired antigen specificity are provided. For example, in certain aspects, methods for determining amino acid sequences of serum antibody CDR3 and abundancy levels are described. In some aspects, methods for determining nucleic acid sequences of antibody variable region sequences and the frequency thereof in biological samples are provided. Furthermore, the invention provides methods for identification and generation of antibodies or antigen-binding fragments that comprise highly-represented CDR domains.
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