MATERIALS AND METHODS FOR PROFILING MICRORNAS

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United States of America Patent

APP PUB NO 20160160274A1
SERIAL NO

14962984

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Abstract

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The present invention provides materials and methods for detecting, quantifying, and/or high-throughput-profiling microRNAs. Advantageously, the present invention is more sensitive and specific than other currently-available miRNA qPCR assays. In addition, the present invention is convenient, easy-to-perform, and cost-effective. In one embodiment, the present invention provides a universal primer for reverse transcription of all miRNAs, a universal reverse primer for PCR amplification reaction, and universal probes. In another embodiment, the present invention provides assays that allow simultaneous detection and/or quantification of a plurality of target miRNAs using a single reverse transcription reaction.

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Patent Owner(s)

Patent OwnerAddress
UNIVERSITY OF SOUTH FLORIDATAMPA FL

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
MOHAPATRA, SHYAM S LUTZ, US 99 499
WANG, JIA-WANG TAMPA, US 20 74

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