Methods of Embryogenic Tissue Preparation for Sugar Cane Transformation
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United States of America Patent
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Jun 16, 2016
app pub date -
Dec 12, 2014
filing date -
Dec 12, 2014
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Abstract
Methods of tissue preparation for the transformation of sugar cane are provided. The methods comprise a pre-culture treatment of sugar cane embryogenic tissue prior to transformation. The methods comprise excising a segment of plant tissue from a shoot of sugar cane; culturing said segment to produce sugar cane embryogenic tissues; performing a pre-culturing treatment by sub-culturing responding embryogenic tissue on fresh media for a period of time of at least 7 days, in the same media; and with no intervening step and no changes of media, performing transformation of embryogenic tissue. Transformation can be performed via Agrobacterium-mediated gene delivery, biolistic transformation, and the like. Transgenic plants are regenerated from plantlets grown under conditions favoring growth of transformed cells while substantially inhibiting growth of non-transformed cells.
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