IMMEDIATE CHROMATIN IMMUNOPRECIPITATION AND ANALYSIS
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United States of America Patent
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Jun 16, 2016
app pub date -
Dec 10, 2015
filing date -
Dec 10, 2014
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Abstract
The present invention relates to a newly developed immediate chromatin immunoprecipitation procedure (“ZipChIP”). ZipChIP significantly reduces the time and increases sensitivity allowing for rapid screening of multiple loci. ZipChIP enables the detection of histone modifications (e.g., H3K4 mono- and trimethylation) and at least two yeast histone demethylases, Jhd2 and Rph1, which were previously found difficult to detect using standard methods. ZipChIP further relates to the enrichment of the histone deacetylase Sir2 at heterochromatin in yeast and enrichment of the chromatin remodeler, PICKLE, in Arabidopsis thaliana.
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