USE OF MULTIPLE RECOMBINATION SITES WITH UNIQUE SPECIFICITY IN COMBINATIONAL CLONING

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United States of America Patent

APP PUB NO 20160222379A1
SERIAL NO

14846344

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ATTORNEY / AGENT: (SPONSORED)

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Abstract

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The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites with unique specificity. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different. Such molecules and/or compounds or combinations of such molecules and/or compounds can also be bound through recombination to various structures or supports according to the invention.

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Patent Owner(s)

Patent OwnerAddress
LIFE TECHNOLOGIES CORPORATION5823 NEWTON DRIVE CARLSBAD CA 92008

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
BRASCH, MICHAEL GAITHERSBURG, US 14 19
BYRD, DEVON FREDERICKSBURG, US 6 21
CHEO, DAVID KESINGTON, US 22 407
HARTLEY, JAMES FREDERICK, US 13 10
TEMPLE, GARY WASHINGTON GROVE, US 10 8

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