METHOD FOR CAPTURING AND ENCODING NUCLEIC ACID FROM A PLURALITY OF SINGLE CELLS
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United States of America Patent
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Aug 25, 2016
app pub date -
Sep 29, 2014
filing date -
Sep 30, 2013
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Abstract
This invention relates to methods for capturing and encoding nucleic acid from a plurality of single cells. A plurality of solid supports is randomly placed into a plurality of compartments, such that the average number of solid supports per compartment, λ1, is less than 1, wherein each solid support carries (a) a unique identification sequence and (b) a capture moiety. A plurality of single cells is randomly placing into the plurality of compartments, such that the average number of cells per compartment, λ2, is less than 1. These random placement steps may be performed in any order. Nucleic acid is then released from each single cell and captured via the capture moiety, such that nucleic acid from each single cell is tagged with a unique identification sequence.
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