METHOD FOR GENOME SEQUENCING USING A SEQUENCE-BASED PHYSICAL MAP
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United States of America Patent
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Sep 1, 2016
app pub date -
Feb 3, 2016
filing date -
Jul 12, 2006
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Abstract
The present invention relates to a high throughput method for the identification and detection of molecular markers wherein restriction fragments are generated and suitable adaptors comprising (sample-specific) identifiers are ligated. The adapter-ligated restriction fragments may be selectively amplified with adaptor compatible primers carrying selective nucleotides at their 3′ end. The amplified adapter-ligated restriction fragments are, at least partly, sequenced using high throughput sequencing methods and the sequence parts of the restriction fragments together with the sample-specific identifiers serve as molecular markers.
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