FLUORESCENT PROTEIN BIOSENSORS TO PROBE THE CONFORMATIONAL ACTIVATION OF CDK/CYCLIN KINASES
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United States of America Patent
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Oct 6, 2016
app pub date -
Nov 13, 2013
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Nov 13, 2013
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Abstract
The present invention relates to compounds comprising a polypeptide comprising an amino acid sequence derived from the sequence of a cyclin-dependent kinase (CDK) and at least one fluorophore coupled to an amino acid of said polypeptide. The invention also relates to said compounds associated with a technology enabling their intracellular delivery. The present invention also relates to the use of such compounds, or of compositions comprising said compounds, for determining if a product is capable of modulating the conformation of a CDK. More particularly, a compound according to the invention is usable for high-content high-throughput screening.
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