Compositions and methods for performing hybridizations with separate denaturation of the sample and probe

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United States of America Patent

PATENT NO 10202638
APP PUB NO 20160304945A1
SERIAL NO

15096036

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Abstract

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The invention provides methods and compositions for separately denaturing a probe and target in hybridization applications. The invention may, for example, eliminate the use of or reduce the dependence on formamide in hybridization applications. Compositions for use in the invention include an aqueous composition comprising at least one polar aprotic solvent in an amount effective to denature double-stranded nucleotide sequences.

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Patent Owner(s)

  • DAKO DENMARK A/S

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Matthiesen, Steen Hauge Hillerod, DK 27 238

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