COMPOSITIONS AND METHODS FOR NUCLEIC ACID AMPLIFICATION
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United States of America Patent
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Nov 17, 2016
app pub date -
May 10, 2016
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May 11, 2015
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Abstract
The present disclosure provides compositions, methods and systems for the efficient removal of a non-target nucleic acid sequence from a double stranded nucleic acid amplification product. A non-target nucleic acid sequence may be a primer sequence incorporated into the double stranded nucleic acid molecule during a nucleic acid synthesis or amplification reaction. The non-target nucleic acid sequence may include a nucleobases that are not canonical DNA nucleobases, such as uracil, that can be selectively removed as part of the non-target sequence removal process.
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