NANOCARRIER BASED PLANT TRANSFECTION AND TRANSDUCTION
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United States of America Patent
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Issued Date -
Nov 24, 2016
app pub date -
Jun 2, 2016
filing date -
Jun 7, 2007
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Abstract
The present invention provides a novel method for the transduction and/or transfection of plant cells. Cell-penetrating peptides (CPPs) have been successfully employed as nanocarriers to deliver proteins and oligonucleotides to single plant cell microspores as well as multi-cellular zygotic embryos. The efficiency of CPP internalization and further delivery of a macromolecular cargo comprising a protein and/or an oligonucleotide can be enhanced by permeabilization of the zygotic embryos.
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