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Abstract

Methods of detecting RNA, such as ribosomal RNA (rRNA), messenger RNA (mRNA), and others. The methods include heating a cell comprising RNA in a solution to release the RNA from the cell, reverse transcribing the RNA into DNA with an enzyme, amplifying the DNA with the same enzyme, and detecting the amplified DNA. The heating, reverse-transcribing, and amplifying in at least some of the methods are performed at substantially the same temperature and a substantially constant temperature without adding additional reagents during or between the steps. The methods can be used to detect the presence of one cell type as distinguished from another cell type within a sample or to determine levels of gene expression, each without the need for elaborate extraction protocols.

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First Claim