DEFINED THREE DIMENSIONAL MICROENVIRONMENT FOR CELL CULTURE
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United States of America Patent
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Dec 8, 2016
app pub date -
Jun 3, 2016
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Jun 5, 2015
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Abstract
This disclosure provides a three-dimensional (3D) microenvironment presenting defined biochemical and physical cues that regulate cellular behavior and use of the microenvironment. A composition to form the 3D microenvironment is provided by combining one or more natural or synthetic polymeric materials and substrate proteins recombinantly or chemically functionalized with a variety of bioactive peptides such as extracellular matrix-derived or growth factor-derived peptides. The disclosure also provides for devices and methods for screening for optimal combinations of the bioactive motifs in order to create an extracellular microenvironment that can regulate specific cellular behavior such as cell growth, proliferation, migration or differentiation.
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