METHOD AND SYSTEM FOR SEQUENCING IN CHARACTERIZATION OF ANTIBODY BINDING BEHAVIOR
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United States of America Patent
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Issued Date -
Dec 15, 2016
app pub date -
Jun 15, 2016
filing date -
Jun 15, 2015
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Abandoned
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Abstract
A method and system for characterization of antibody-bound targets by sequencing of synthetic oligonucleotides bound to the antibodies, the method including: receiving a sample having a set of antibodies; conjugating each of the set of antibodies with an oligonucleotide, thereby generating a set of oligonucleotide-conjugated antibodies; binding a first subset of the set of oligonucleotide-conjugated antibodies to a set of targets at a capture substrate; determining a sequence for at least one of: 1) each oligonucleotide of the first subset of oligonucleotide-conjugated antibodies that bind to the set of targets and 2) each oligonucleotide of a second subset of the set of oligonucleotide-conjugated antibodies that fail to bind to the set of targets; and generating an analysis of the sample from the sequences determined from at least one of the first and the second subsets of oligonucleotide-conjugated antibodies.
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