SCHIFF-BASE CONJUGATE OF N, N-DIBUTYL-P-PHENYLENEDIAMINE WITH PYRIDOXAL 5'-PHOSPHATE FOR IMPROVED HOMOCYSTEINE ASSAYS USING PYRIDOXAL 5'-PHOSPHATE-DEPENDENT ENZYMES
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United States of America Patent
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Sep 9, 2016
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Jan 10, 2013
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Abstract
A composition, method and kit for performing a two-reagent enzymatic homocysteine assay, wherein a single homocysteinase enzyme and a Schiff-based conjugate of N,N-dibutyl-p-phenyldiamine (DBPDA) with pyridoxal 5′-phosphate (PLP) are used to measure total homocysteine in plasma or serum. The assay measures a chromophore reaction product of H2S and the DBPDA released from the Schiff-base conjugate in the presence of a Fe+3 containing compound. The resulting chromophore may be measured absorbance or fluorescence spectrophotometry.
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