Importance

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Abstract

A gas recirculation system for an incubated controlled environment, the system comprising: a first mixing box; a second mixing box in fluid communication with the first mixing box; a manifold in fluid communication with the second mixing box; a plurality of incubation chambers in fluid communication with the manifold; a return manifold in fluid communication with the plurality of incubation chambers and in fluid communication with the first mixing box. A gas recirculation system for an incubated controlled environment, the system comprising: a first mixing box; a second mixing box in fluid communication with the first mixing box; an inlet port in fluid communication with the second mixing box; a plurality of incubation chamber inlet ports; an incubation chamber in fluid communication with the plurality of incubation chamber inlet ports; a plurality of incubation chamber outlet ports in fluid communication with the incubation chamber; a check valve in fluid communication with the plurality of incubation outlet ports, and in fluid communication with the second mixing box. A gas recirculation system for an incubated controlled environment, the system comprising: a pump; a filter in fluid communication with the pump; a sensor box in fluid communication with the filter; a pH sensor box in fluid communication with the sensor box, the pH sensor box comprising a container with embryo culture media and a pH sensor configured to determine the pH of the embryo culture media; a regulator in fluid communication with the pH sensor box; an embryo chamber in fluid communication with the regulator; a second pH sensor box in fluid communication with the regulator, the pH sensor box comprising a container with embryo culture media and a pH sensor configured to determine the pH of the embryo culture media. A gas recirculation system for an incubated controlled environment, the system comprising: a means for monitoring the O2 and CO2 concentration of gases in the incubated controlled environment; a means for injecting CO2, N2, and/or O2 into the incubated controlled environment via a mixing box; a means for adjusting the CO2, N2, and O2 levels in the incubated controlled environment. A gas recirculation system for an incubated controlled environment of claim 16, further comprising: a means for introducing CO2, N2, and/or O2 into the incubated environment in order to maintain about 5% of CO2; about 90% of N2; and/or about 5% of O2 in the incubated environment.

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First Claim