ACCURATE DETECTION OF RARE GENETIC VARIANTS IN NEXT GENERATION SEQUENCING
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United States of America Patent
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Jan 19, 2017
app pub date -
Mar 18, 2015
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Mar 28, 2014
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Abstract
The invention relates to a method for analyzing a target nucleic acid fragment, comprising generating a first strand using one strand of the target as a template by primer extension, using a first oligonucleotide primer which comprises, from 5′ to 3′, an overhang adaptor region, a primer ID region and a target specific sequence region complementary to one end of the target fragment; optionally removing non-incorporated primers; amplifying the target from the generated first strand to produce an amplification product; and detecting the amplification product. Also disclosed are unique primers useful for such target analysis methods.
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