Optimizing operating binding capacity for a multiple column chromatography process

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United States of America Patent

PATENT NO 11835501
APP PUB NO 20170016864A1
SERIAL NO

14797785

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Abstract

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Determining optimum operating binding capacity for a MCC process using one column, the process including N number of columns, comprises

    loading target product on a first column at a first residence time and/or flow rate;loading the product on the column at a second residence time and/or flow rate, the first residence time and/or flow rate being different than the second residence time and/or flow rate;generating a first breakthrough curve for the first residence time and/or flow rate and a second breakthrough curve for the second residence time and/or flow rate, wherein the first curve represents product breakthrough for the first column and the second curve represents product breakthrough for an Nth column; andusing the curves to determine product loading capacity of the first column before product breakthrough at the Nth column; the product loading capacity of the first column equaling optimum operating binding capacity for the process.

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Patent Owner(s)

  • SARTORIUS STEDIM CHROMATOGRAPHY SYSTEMS LTD.

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Gjoka, Xhorxhi Worcester, US 3 5
Rogler, Karl K Lincoln, US 4 6
Schofield, Mark J N. Grafton, US 2 5

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