CULTURE MEDIUM AND CULTURING METHOD FOR ANCHORAGE-DEPENDENT CELLS, CELL COMPOSITION INCLUDING STEM CELLS AND/OR DIFFERENTIATED CELLS DERIVED FROM STEM CELLS, AND PRODUCTION METHOD FOR CELL COMPOSITION

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United States of America Patent

SERIAL NO

15313861

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Abstract

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As a technique capable of culturing anchorage-dependent cells without using an anchorage, provided is a medium for anchorage-dependent cells, which comprises MFG-E8 (Milk fat globule-EGF factor 8) or a fragment of the protein. This medium can promote adhesion of anchorage-dependent cells in the absence of an anchorage, enables the survival and proliferation (colony formation) of the cells, and further, also enables the subsequent differentiation if the anchorage-dependent cells are stem cells.

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Patent Owner(s)

Patent OwnerAddress
KYOWA HAKKO KIRIN CO LTDTOKYO 100-8185

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
NAKASHIMA, Yoshiki Osaka, JP 46 565
OMASA, Takeshi Osaka, JP 7 55
TSUKAHARA, Masayoshi Tokyo, JP 11 15

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