Thermophilic DNA polymerase mutants

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United States of America Patent

PATENT NO 10626383
SERIAL NO

15405574

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Abstract

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This disclosure relates to thermophilic family B DNA polymerases comprising a neutral amino acid residue at a certain position near the C-terminus of the catalytic domain, which corresponds to a position occupied by a basic amino acid residue in wild-type Pfu polymerase. Related uses, methods, and compositions are also provided. In some embodiments, the polymerases comprise a 3′-5′ exonuclease domain and/or a sequence non-specific dsDNA binding domain.

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Patent Owner(s)

Patent OwnerAddress
THERMO FISHER SCIENTIFIC BALTICS UAB5823 NEWTON DRIVE CARLSBAD CA 92008

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Matjosaitis, Karolis Vilnius, LT 3 3
Rimseliene, Renata Vilnius, LT 7 22
Simutyte, Martyna Vilnius, LT 2 3
Skirgaila, Remigijus Vilnius, LT 12 126
Sukackaite, Rasa Vilnius, LT 7 3
Ukanis, Mindaugas Vilnius, LT 5 95
Valinskyte, Skaiste Vilnius, LT 5 3

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