Importance

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Abstract

At least one nucleic acid from a sulphate-reducing bacteria may be extracted from an oilfield fluid and may be amplified by a PCR amplification method in the presence of at least one primer to form an amplification product. The primer(s) may be or include a sequence essentially identical to SEQ ID NO:1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, and mixtures thereof. The amplification product may be hybridized with a probe specific for a fragment of an alpha subunit of an APS gene, and a presence of hybridization and a degree of hybridization may be detected.

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First Claim