METHOD FOR PRODUCING GAMMA DELTA T CELLS, AND PHARMACEUTICAL THEREOF
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United States of America Patent
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Oct 13, 2015
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Oct 14, 2014
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Abstract
The purpose of the present invention is to provide a method for producing γδT cells with which it is possible to obtain a cell population including a large amount of γδT cells and including virtually no cells infected by human T-cell leukemia virus (HTLV) even when PBMCs derived from a person infected with HTLV are used as the cell source. A cell population including a large amount of γδT cells and including virtually no HTLV-1-infected cells is obtained by culturing after removing CD4-positive cells that express CD4 (referred to hereinafter as “CD4+ cells”) and CD8-positive cells that express CD8 (referred to hereinafter as “CD8+ cells”) from the PBMCs that are used as the cell source, or after removing cells that express αβT cell receptors (αβTCR) (referred to hereinafter as “αβT cells”), before expanding γδT cells.
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