ULTRAFILTRATION FOR PREPARING OUTER MEMBRANE VESICLES

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United States of America Patent

APP PUB NO 20170252699A1
SERIAL NO

15599556

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Abstract

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In place of a step of centrifugation during preparation of outer membrane vesicles (OMVs) from bacteria, the invention utilises ultrafiltration. This allows much larger amounts of OMV-containing supernatant to be processed in a much shorter time. Thus the invention provides a process for preparing bacterial OMVs, comprising a step of ultrafiltration. The ultrafiltration step is performed on an aqueous suspension of OMVs after they have been prepared from bacteria and the OMVs remain in suspension after the filtration step. The invention is particularly useful for preparing OMVs from Neisseria meningitidis.

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Patent Owner(s)

Patent OwnerAddress
GLAXOSMITHKLINE BIOLOGICALS S A1330 RIXENSART

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
MARSILI, Ilio Siena, IT 2 7
OLIVIERI, Roberto Siena, IT 19 106
SABBATINI, Fabio Siena, IT 7 26

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