Method for estimating the probe-target affinity of a DNA chip and method for manufacturing a DNA chip

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United States of America Patent

PATENT NO 10964407
APP PUB NO 20170270242A1
SERIAL NO

15532220

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Abstract

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A method for estimating the affinity ϕ of a first DNA strand, or “probe”, to be hybridized with a second DNA strand, or “target”, to form a hybrid of length Lbp, the method comprising: in each division of a set of M divisions of the hybrid, counting the number of times in which each hybrid of a set of P DNA strand hybrids is present in the division, the hybrids being of length k less than the length Lbp, or “k-hybrids”; for each combination of mismatches of a set of L combinations of mismatches in a hybrid of length Lbp, determining whether the pair of mismatches is present in the hybrid; and calculating the affinity ϕ according to the relation:

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Patent OwnerAddress
BIOMERIEUX376 CHEMIN DE L'ORME MARCY-L'ÉTOILE 69280
HOSPICES CIVILS DE LYON3 QUAI DES CELESTINS LYON 69002

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Becker, Jérémy Chartres, FR 1 0
Mallet, François Villeurbanne, FR 21 10
Perot, Philippe Saint-Yrieix, FR 12 12

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