PRODUCTION METHOD FOR NERVE TISSUE
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United States of America Patent
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Oct 23, 2015
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Oct 24, 2014
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Abstract
The present invention provides a method for producing neural cells or a neural tissue, including the following steps (1)-(3):
- (1) a first step of culturing pluripotent stem cells in the absence of feeder cells and in a medium containing 1) a TGFβ family signal transduction pathway inhibiting substance and/or a Sonic hedgehog signal transduction pathway activating substance, and 2) a factor for maintaining undifferentiated state,(2) a second step of culturing the cells obtained in the first step in suspension to form a cell aggregate, and(3) a third step of culturing the aggregate obtained in the second step in suspension in the presence or absence of a differentiation-inducing factor to obtain an aggregate containing neural cells or a neural tissue.
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