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Abstract

The present invention provides a novel method for more simply and rapidly detecting target bacterial cells by a device using a binding molecule capable of binding to the target bacterial cells. In the method, a sample is incubated in a reagent for concentration of bacteria to cause the sample to react with a fluorescently-labeled binding molecule, and a fluorescence polarization degree is then detect to detect the target, and this is performed using a bacterial detection tool. The bacterial detection tool is obtained by attaching a sample collection tool to a main body. The sample collection tool includes a collection section and a connection section that is connected to the main body. The main body includes a reagent storage chamber, a collection section storage chamber, and a connection section that is connected to the sample collection tool. The reagent storage chamber and the collection section storage chamber are separated from each other. The sample collection tool and the main body are connected to each other at the connection section of the sample collection tool and the connection section of the main body after the preparation step and before the incubation step with the collection section being placed inside the collection section storage chamber. The reagent storage chamber and the collection section storage chamber internally communicate with each other after the preparation step to perform the incubation step and the reaction step.

h a sample is collected; and a connection section that is connected to the main body,the main body comprises:a reagent storage chamber that contains the reagent and the fluorescently-labeled binding molecule;a collection section storage chamber that contains the collection section of the sample collection tool; anda connection section that is connected to the sample collection tool,the reagent storage chamber and the collection section storage chamber are separated from each other, andthe sample collection tool and the main body are connected to each other at the connection section of the sample collection tool and the connection section of the main body with the collection section of the sample collection tool being placed inside the collection section storage chamber of the main body.

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First Claim