Method for obtaining high-yield, stable expression cell clones and antibody molecules obtained thereby

Number of patents in Portfolio can not be more than 2000

United States of America Patent

PATENT NO 10457747
APP PUB NO 20180016353A1
SERIAL NO

15549087

Stats

ATTORNEY / AGENT: (SPONSORED)

Importance

Loading Importance Indicators... loading....

Abstract

See full text

Provided is a method for obtaining high-yield, stable expression cell clones from myeloma cell lines in a protein-free culture medium. The method is used for industrial production of a recombinant antibody, and includes three stage: (1) adapting to a protein-free culture medium, statically culturing cells at a low density, and gradually reducing a fat-rich supplement to a chemical culture medium; (2) adapting to a protein-free culture medium; culturing cells at a high density, and using a perfusion fermentation system in a laboratory scale; and (3) screening high-yield, stable expression cell clones from the cells after fermentation ends. The cell clone may be used to produce a humanized anti-NeuGcGM3 14F7 recombinant antibody.

Loading the Abstract Image... loading....

First Claim

See full text

Family

Loading Family data... loading....

Patent Owner(s)

  • BIOTECH PHARMACEUTICAL CO. LTD.; CENTRO DE INMUNOLOGIA MOLECULAR

International Classification(s)

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Arias, Miguel Havana, CU 5 12
Bai, Xianhong Beijing, CN 4 58
Bai, Zhi Shanxi Province, CN 1 0
Cai, Yangliu Beijing, CN 1 0
Calvo, Loany Havana, CU 1 0
Chea, MeyLen Havana, CU 1 0
Chen, Xiao Henan Province, CN 265 1345
González, Tamara Havana, CU 1 0
He, Zhenhua Beijing, CN 5 1
Liu, Yuemao Hunan Province, CN 1 0
Pérez, Rolando Havana, CU 1 0
Palacios, Julio Havana, CU 1 0
Xiao, Kaiheng Beijing, CN 1 0
Yang, Zhenhua Shan Xi Sheng, CN 10 94

Cited Art Landscape

Load Citation

Patent Citation Ranking

Forward Cite Landscape

Load Citation

Maintenance Fees

Fee Large entity fee small entity fee micro entity fee due date
7.5 Year Payment $3600.00 $1800.00 $900.00 Apr 29, 2027
11.5 Year Payment $7400.00 $3700.00 $1850.00 Apr 29, 2031
Fee Large entity fee small entity fee micro entity fee
Surcharge - 7.5 year - Late payment within 6 months $160.00 $80.00 $40.00
Surcharge - 11.5 year - Late payment within 6 months $160.00 $80.00 $40.00
Surcharge after expiration - Late payment is unavoidable $700.00 $350.00 $175.00
Surcharge after expiration - Late payment is unintentional $1,640.00 $820.00 $410.00