Gene knockout method

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United States of America Patent

PATENT NO 10863730
APP PUB NO 20180020646A1
SERIAL NO

15538554

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Abstract

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A method for producing a cell in which a target gene is knocked out, the method including the step of: introducing a CRISPR-Cas system into a cell having one or more kinds of target genes, the CRISPR-Cas system being able to produce (i) three or more kinds of guide RNAs for each of the one or more kinds of target genes and (ii) a Cas protein. The present invention can provide a method that enables highly efficient (90% or more) production of whole-body biallelic knockout animals in a single generation.

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Patent Owner(s)

Patent OwnerAddress
RIKENWAKO-SHI SAITAMA 351-0198

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Perrin, Dimitri Saitama, JP 1 6
Sumiyama, Kenta Saitama, JP 3 9
Sunagawa, Genshiro Saitama, JP 3 6
Ueda, Hiroki Saitama, JP 100 685
Ukai, Maki Saitama, JP 1 6

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