Importance

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Abstract

The present invention provides an incubator with a clean bench function to have both function of opened and closed system and a proposal how to use, allowing cells or others to be “closable and openable” manipulated and cultured, as well as an incubator, a globe box, a clean bench. This is implemented in this system has a specific structure and a characteristic component, e.g., a structure classified two units by the inside temperature, an operation cover and a circular fixture, a channel from/to inside or outside etc. to allow such as used, a non-opening operation and supply and observation from outside, or a moving of perfusion culture device and medium storing bags kept on connecting tubes or others. An incubator 100 with a clean bench function mainly includes a chamber 110, 210, 220, 230, a temperature control unit 160, 213 and a gas concentration control and supply unit 140, 240, as well as a CO₂ cylinder 170, a N₂ cylinder 180, and a N₂ gas generator 190. The chamber 210 has an ability of setting on low temperature. The chamber 110 has the shape of a rectangular parallelepiped and includes an opening 111 in the front, and a sensor 161. The opening 111 attached on a sealing door 117 and an inner door 118, and their intervening space with elastic materials 120-122 and channels 126-128 in a peripheral portion 112 around the opening 111. For the closable operation inside the chamber 110 from the outside, an operation cover 136 and a circular fixture 300, a membrane with/without slit 134 and the fixer 134a, b is attached on/off the fixing hole 119 in the inner door 118, and a first hook 114 to hang of the operation cover is attached to each of two inner side surfaces 113 of the chamber 110. A UV lamp 116 adapted to emit UV rays for sterilization is attached on upper site of inner back surface 115 in the chamber 110. The N₂ gas supplied in large quantity into the chamber from N₂ cylinder 180 that positive pressure is maintained in the chamber 110 relative to outside pressure as used the clean bench function. In particular, this present invention is possible to mostly three new matters as follows: First, the exchange and regulate with gas composition and temperature in this system is both of them different constituent at the same time. Second, this system is an ability to operate under the condition of very low temperature (from 4 degree Celsius) and low O₂ concentration (from 1%), too. Third, this system is an ability applied to variety of opened and closed operation etc. under the keeping with condition.

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First Claim