CONTRACTILE CELLULAR CONSTRUCT FOR CELL CULTURE
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United States of America Patent
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May 16, 2016
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May 15, 2015
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Abstract
The present invention relates to a method for producing a contractile cellular construct, comprising the steps of: a) seeding pre-selected cells onto a mold, wherein the pre-selected cells comprise signal emitting agents; and b) culturing the pre-selected cells to produce the cellular construct. The said method is exemplified by mixing cardiomyocytes with fluorescent microbeads, seeding the suspension on a polydimethylsiloxane (PDMS) mold and culturing to form a contractile device comprising of cardiac muscles. The present invention also relates to a method for measuring the contractility of the cellular construct by measuring the displacement of the signal emitting agents, and a method for screening a method for screening one or more agents for modulating the contractility of a contractile cellular construct.
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