Cleavable hairpin primers

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United States of America Patent

PATENT NO 10858699
APP PUB NO 20180057868A1
SERIAL NO

15689084

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Abstract

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The invention describes composition and methods of use for novel hairpin blocked-cleavable primers. In one embodiment unblocking occurs through action of RNase H2. The method improves the specificity of PCR and reduces primer dimer events, enabling higher level multiplex reactions. Additionally, the invention protects RNA-containing primers from attack by single-strand RNases.

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Patent Owner(s)

Patent OwnerAddress
INTEGRATED DNA TECHNOLOGIES INC1710 COMMERCIAL PARK CORALVILLE IA 52241

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Behlke, Mark Aaron Coralville, US 74 956
Dobosy, Joseph Coralville, US 25 239
Walder, Joseph A Chicago, US 24 1497

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