METHODS OF PURIFYING ADENO-ASSOCIATED VIRUS (AAV) AND/OR RECOMBINANT ADENO-ASSOCIATED VIRUS (rAAV) AND GRADIENTS AND FLOW-THROUGH BUFFERS THEREFORE
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Nov 7, 2017
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Jan 13, 2015
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Abstract
A method of purifying a viral vector is provided. The method includes loading a nonionic density gradient into a continuous flow centrifuge rotor; loading a material including the viral vector and a predictable contaminant into the continuous flow centrifuge rotor; rotating the continuous flow centrifuge rotor in a manner sufficient to separate the viral vector from the predictable contaminant and the material in the nonionic density gradient; and unloading the separated viral vector from the continuous flow centrifuge rotor.
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