Determination of cells using amplification

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United States of America Patent

PATENT NO 10876156
APP PUB NO 20180066305A1
SERIAL NO

15557622

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Abstract

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The present invention generally relates to microfluidics and, in particular, to systems and methods for determining cells using amplification. In one set of embodiments, cells are encapsulated within droplets and nucleic acids from the cells amplified within the droplets. The droplets may then be pooled together and the amplified nucleic acids can be determined using PCR or other suitable techniques. In some embodiments, techniques such as these can be used to detect relatively rare cells that may be present, e.g., if the droplets are amplified using conditions able to selectively amplify nucleic acids arising from the relatively rare cells.

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Patent Owner(s)

  • PRESIDENT AND FELLOWS OF HARVARD COLLEGE

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Weitz, David A Bolton, US 195 10066
Zhang, Huidan Cambridge, US 13 48

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